We narrowed to 2,376 results for: 683

1. pDY118A

   Plasmid

   #182958

   Purpose

   pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Inserts

   Cas9
   Gam, Beta, Exo
   I-scel
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

   Plasmid

   #163881

   Purpose

   Acute Depletion of Mau2-mScarlet

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Mau2 (Mau2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

   Plasmid

   #52521

   Purpose

   Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Insert

   ChR2-2a-hM4D
   

   Use

   AAV and Cre/Lox

   Tags

   2xHA on ChR2

   Expression

   Mutation

   Promoter

   CBA

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV_VSVgSS-diCR2

   Plasmid

   #207324

   Purpose

   Expression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infection

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   diCR2 (LDLR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fragment of LDL-R dimerized

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEBB COMMD1 Flag

   Plasmid

   #74893

   Purpose

   Express COMMD1 in mammalian cells

   Depositor

   Ezra Burstein, Colin Duckett

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD1 (COMMD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMx/ATP11C-HA

   Plasmid

   #209237

   Purpose

   Mammalian expression of ATP11C

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR4-huNFATc1nuc

   Plasmid

   #23988

   Depositor

   Jerry Crabtree

   Article

   Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

   Insert

   NFATc1 (NFATC1 Human)
   

   Use

   Cloning vector

   Tags

   EE tag

   Expression

   Mutation

   S172A S175A S176A S178A S179A S181A S184A S187A S…

   Promoter

   Available since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT82_GalL_nCDA1Δ198-BE3

   Plasmid

   #145046

   Purpose

   Expresses nCDA1Δ198-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ198-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-198AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPR v2 mBAK

    Plasmid

    #129578

    Purpose

    CRISPR deletion of murine BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAK (Bak1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-BBS4

    Plasmid

    #218713

    Purpose

    Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS4 (BBS4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I354T (t1110c)

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-TetO-Dppa2

    Plasmid

    #40799

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

    Insert

    Dppa2 (Dppa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV + TetO

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEAK-cKal7

    Plasmid

    #145801

    Purpose

    expresses Kalirin-7 with the c-front in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    cKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pJT25_GalL_BE3

    Plasmid

    #143736

    Purpose

    Expresses BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCerulean3-Golgi-7

    Plasmid

    #55420

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

17. HA, EGFP Spastin M87 with exon 4, N386C

    Plasmid

    #167543

    Purpose

    For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

    Depositor

    Tarun Kapoor

    Article

    Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

    Insert

    Spastin M87 with exon 4 (SPAST Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    N386C

    Promoter

    CMV

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mApple-M11-92

    Plasmid

    #134466

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX GFP cdc42 Q61L

    Plasmid

    #14568

    Depositor

    Joan Brugge

    Article

    Hall et al Immunity. 2006 Mar . 24(3):305-16.

    Insert

    cdc42 Q61L (CDC42 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only