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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PLD1

Plasmid

#45268

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PLD1 (PLD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH2

Plasmid

#129423

Purpose

Expression human MSH2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH2 (MSH2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Nicn1

Plasmid

#92344

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 delta-F

Plasmid

#81116

Purpose

mammalian expression of F-box deleted Skp2

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deleted F box

Promoter

CMV

Available Since

Sept. 24, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Nrxn1beta AS4(+)-Fc

Plasmid

#59314

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-FOXP2

Plasmid

#47053

Purpose

Expresses forkhead box P2

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Forkhead Box P2 (FOXP2 Human)

Use

Gateway Cloning

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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5xNF-kb_RE::RedF

Plasmid

#124530

Purpose

NFKB RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

NF-κβ RedF reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAT001

Plasmid

#171061

Purpose

Plasmid for expressing HIV-1 envelope glycoprotein

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

HIV-1 Env

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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