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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,766 results for: loxP

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Showing: 1481 - 1500 of 7766 results

  1. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid
    #85072
    Purpose
    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    Tags
    6xHis and mScarlet-i and SGFP2
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Q63L
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85072-plasmid-map-sequence-id-163878

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  6. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Expression
    Insect
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

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  15. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Publication
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hUBC promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

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  16. pAAV-TRE-FLEX-ChR2-YFP

    Plasmid
    #110337
    Purpose
    ChR2-YFP reporter vector of iTango and Cal-Light system for AAV production
    Depositor
    Hyungbae Kwon
    Insert
    tetO-Flex-ChR2-YFP
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Chr2 H134R
    Promoter
    tetO
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110337-plasmid-map-sequence-id-211135

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  18. iF59 PB-TO-oNgn2-puro

    Plasmid
    #204734
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Insert
    Optimized neurogenin-2
    Expression
    Mammalian
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    204734-plasmid-map-sequence-id-403386

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  19. pYC-N

    Plasmid
    #207113
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Publication
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Oct. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    207113-plasmid-map-sequence-id-410641

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Showing: 1481 - 1500 of 7766 results