Addgene

We narrowed to 3,104 results for: pcdna3 1

Showing: 1481 - 1500 of 3104 results

Results per page:

Sort results by:

Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Use

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+) EGFP-Rac1(P29S)

Plasmid

#224280

Purpose

Cell transfection and expression of Rac1 (P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

P29S

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Use

Tags

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+) EGFP-Rac1(G12C)

Plasmid

#224279

Purpose

Cell transfection and expression of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LARG mtq sspB in pcDNA3.1

Plasmid

#90460

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mTq
sspB

Use

Tags

Expression

Mammalian

Mutation

R73Q and deletion a.a. (234-239)

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyso-PTEN(A4)-mCherry

Plasmid

#184054

Purpose

Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-PTEN(A4)-mCherry (PTEN Human)

Use

Tags

Lysosome-associated membrane protein 1 (LAMP1). a…

Expression

Mammalian

Mutation

Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CMV-Grid2(I677C)

Plasmid

#164782

Purpose

cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

Insert

Grid2 I677C (Grid2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

I677C

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flt3-Y591F/Y919F

Plasmid

#74497

Purpose

Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-S586A-VLCAD-FLAG

Plasmid

#55824

Purpose

Expresses S586A VLCAD mutant in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

changed Serine 586 toAlanine

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-RasAR(R89L)-NES

Plasmid

#205568

Purpose

RasAR negative-control mutant plasmid. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR(R89L)-NES (RAF1 Synthetic, Human)

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Arg 89 mutated to Leu in Raf1 RBD region.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-RIPK1 K163R

Plasmid

#78844

Purpose

To overexpress RIPK1 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K163R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-RIPK1 K115R

Plasmid

#78843

Purpose

To overexpress RIPK1 K115R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K115R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-S-ectodomain

Plasmid

#235984

Purpose

Internally tagged SARS-CoV-2 spike ecto-domain

Depositor

Syed Saif Hasan

Article

Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

Insert

Spike protein (S SARS-CoV-2)

Use

Tags

8xHis and Twin-Strep tag and internal Twin-Strep …

Expression

Mammalian

Mutation

aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-RIPK1 K316R

Plasmid

#78845

Purpose

To overexpress RIPK1 K316R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K316R

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge