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Plasmid#184772PurposeRed marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2KatushkaExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKC1-2GFP-ter-TRP1
Plasmid#184773PurposeIntegration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertPKC1 (PKC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-RZ-Cas12j2
Plasmid#189782PurposeGolden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-RZ-Cas12j2
Plasmid#189783PurposeGolden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-RZ-Cas12j2
Plasmid#189785PurposeGolden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-RZ-Cas12j2
Plasmid#173927PurposeGolden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-RZ-Cas12j2
Plasmid#173928PurposeGolden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-RZ-Cas12j2
Plasmid#173929PurposeGolden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-RZ-Cas12j2
Plasmid#173930PurposeGolden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB441
Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB419
Plasmid#185089PurposeSwi6L-GFP expressing N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation at aa 273PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB462
Plasmid#185095PurposeNMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::nma111Dnls1Dnls2-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB354
Plasmid#185082PurposeSwi6L-GFP expressin N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-273PromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB416
Plasmid#185087PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB353
Plasmid#185081PurposeSwi6M-GFP expressing N-term 181 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-181PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB484
Plasmid#185132PurposeSWI6-GFP full length under control of GAL1 promotionDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB486
Plasmid#185133PurposeFull length CRM1 fused to binding domain of two-hybrid plasmidDepositorInsertCRM1
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB491
Plasmid#185134PurposeFull length SWI6 fused to activation domain of two-hybrid plasmidDepositorInsertSWI6
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MtrxRFP2
Plasmid#172323Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInserthuman thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
UseTagsHis-tagExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)
Plasmid#164047PurposeCRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA geneDepositorInsertmCherry-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D977A
Plasmid#129674PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD977A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-E848A
Plasmid#129675PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsE848A; AacCas12b is rice codon optimizedExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A
Plasmid#129676PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D951A
Plasmid#129677PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD951A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-E827A
Plasmid#129678PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationE827A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D977A
Plasmid#129680PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationD977A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-E848A
Plasmid#129681PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationE848A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2
Plasmid#136373PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.3
Plasmid#136374PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.3
Plasmid#136377PurposeGateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2.3
Plasmid#136372PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 21-55 eGFP SV40
Plasmid#132653PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 41-55 eGFP SV40
Plasmid#132652PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank1a_pJet1.2
Plasmid#121978Purposefor generating kank1a riboprobes for WISH in zebrafishDepositorInsertkank1a (si:dkey-146l2.1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mdm1_pJet1.2
Plasmid#121977Purposefor generating mdm1 riboprobes for WISH in zebrafishDepositorInsertmdm1 (mdm1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-hPTGS2-2flag
Plasmid#102498PurposeMammalian expression of PTGS2DepositorInsertPTGS2 (PTGS2 Human)
UseTagsFlag-FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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S1m tagged DANCR
Plasmid#138585PurposeFor in vivo taggingDepositorInsertDANCR (DANCR Human)
UseLentiviralTagsS1m tagExpressionMutationPromoterMSCVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ACE2-GFP
Plasmid#154962PurposepcDNA3.1-ACE2-GFPDepositorInsertACE2-GFP (ACE2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-METTL3-PURO
Plasmid#174218PurposeExpresses dCasRx-METTL3 in mammalian cellDepositorInsertdCasRx-METTL3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPRv2-sgEGFP
Plasmid#86153PurposeLentivirus carrying Cas9/CRISPR for cut in GFP (used as control)DepositorInsertEGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT448
Plasmid#139388PurposeBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT450
Plasmid#139390PurposeBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT492
Plasmid#139397PurposeBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT493
Plasmid#139398PurposeBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT494
Plasmid#139399PurposeBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only