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APneg-SNAP25d

Plasmid

#168014

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO10-6SIM

Plasmid

#190248

Purpose

Expression SUMO10-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

10SUMO-6SIM

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pX165-Cas9-HF1

Plasmid

#140565

Purpose

Expresses Cas9-HF1 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N497A R661A Q695A Q926A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPM2212

Plasmid

#167552

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Cosmid

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25c

Plasmid

#168013

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25b

Plasmid

#168012

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G178E

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-R4-uAb

Plasmid

#101801

Purpose

Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Expression

Mammalian

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-A53T

Plasmid

#181722

Purpose

Galactose inducible expression of Alpha-synuclein-A53T

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

A53T

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL50005

Plasmid

#117536

Purpose

Level0 Golden Gate part: c-terminal tag, YFP

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

c-term tag YFP

Use

Synthetic Biology

Mutation

G65S, L68V, A72S, Y203T, L231H

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S345A

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pSTAP-seq_human_spike-in

Plasmid

#125152

Purpose

Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-FUS

Plasmid

#181711

Purpose

Galactose inducible expression of FUS

Depositor

Alejandro Chavez

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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APT3-VAMP7

Plasmid

#168002

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP7-T3'PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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