We narrowed to 7,234 results for: LYC;

1. PRE-Hsp70BbCas9_1.0

   Plasmid

   #190795

   Purpose

   PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   PRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11-PC(75-228)-H6

   Plasmid

   #183779

   Purpose

   Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Use

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-SU(Z)12(624-900)-H6

   Plasmid

   #183772

   Purpose

   Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

   Insert

   SU(Z)12 residues 624-900 (Su(z)12 Fly)
   

   Use

   Tags

   His tag at the C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSunS 1-335

   Plasmid

   #171673

   Purpose

   SunS 1-335 expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30 W96A-GLTP

   Plasmid

   #170734

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

   Insert

   W96A-GLTP (GLTP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W96A

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 mSmoothened NQ4

   Plasmid

   #126407

   Purpose

   Expression of Smoothened NQ4 in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

   Insert

   m-Smoothened (Smo Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Smo glycosylation site NQ4

   Promoter

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 mSmoothened NQ3

   Plasmid

   #126406

   Purpose

   Expression of Smoothened NQ3 in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

   Insert

   m-Smoothened (Smo Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Smo glycosylation site NQ3

   Promoter

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS315-PGK-SEIPIN2

   Plasmid

   #96987

   Purpose

   Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN2 (AT1G29760 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Phosphoglycerate Kinase (PGK) promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MGAT1-GFP

   Plasmid

   #163647

   Purpose

   Expresses GFP tagged MGAT1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5B-FRT-control-APEX

    Plasmid

    #73187

    Purpose

    Expresses "control"-APEX (cytosolic) in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    control-APEX (Nphp3 Mouse, Glycine max)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    changed glycine 2 to alanine

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hDAGLa-V5

    Plasmid

    #87676

    Purpose

    Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Article

    Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

    Insert

    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV PGK1-3xHA

    Plasmid

    #220913

    Purpose

    Expresses PGK1-3xHA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-3xHA (PGK1 Human)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHM024Env

    Plasmid

    #137691

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV hSyn hPGK1-mRuby

    Plasmid

    #220911

    Purpose

    Neuronal specific expression of the fusion construct PGK1-mRuby

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-mRuby (PGK1 Human)
    

    Use

    AAV

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PRE-Hsp70BbCas9_1.2

    Plasmid

    #190796

    Purpose

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. plenti hSyn PGK1-HALO

    Plasmid

    #220910

    Purpose

    Neuronal specific expression of the fusion construct PGK1-HALO

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-HALO (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHM123DEnv

    Plasmid

    #137668

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI.neo-rPAM1-M314I

    Plasmid

    #145802

    Purpose

    expresses catalytically inactive rat PAM-1 in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed methionine 314 to isoleucine to inactivat…

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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