We narrowed to 30,244 results for: PLE;

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pLenti-hPGK-eGFP-SynaptoZip

Plasmid

#122523

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hPGK

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa2-Cas9-GFP

Plasmid

#208050

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa1-Cas9-GFP

Plasmid

#208049

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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CMVp-dsRed2-Triplex-28-gRNA1-28-pA

Plasmid

#55200

Purpose

Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dsRed2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4A(G12V)

Plasmid

#35634

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4A(G12V) (KRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Hygro DEST (w17-1)

Plasmid

#17295

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Puro DEST (w16-1)

Plasmid

#17296

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi; Destination vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-GBP1-C589A

Plasmid

#138090

Purpose

Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

cysteine 589 to alanine (C589A)

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8ChR2 (H134R)eYFP

Plasmid

#89539

Purpose

Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Holloway et al Eur J Neurosci. 2015 Sep;42(6):2271-82.

Insert

ChR2 (H134R)

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRrsGreenF-E

Plasmid

#205764

Purpose

Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

Plasmid

#160566

Purpose

GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E3-E4-En-1)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-5HRE-GFP NeoR

Plasmid

#128960

Purpose

Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

5X HRE of VEGF (VEGFA Human)

Use

Lentiviral

Tags

d2EGFP

Expression

Mammalian

Promoter

minimal CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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