We narrowed to 9,095 results for: Pol;

1. LentiGuide-Puro-IFITM3g1 (BB15)

   Plasmid

   #139460

   Purpose

   Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HP-Cas9hGem-Ade2-LEU2-LINEAR

   Plasmid

   #174839

   Purpose

   pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TDH3p

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2166

   Plasmid

   #158412

   Purpose

   High Copy number plasmid containing Ple331 (PAX6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple331 (PAX6 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple331

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. GST-PBD H538A/K540M

   Plasmid

   #60879

   Purpose

   Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

   Depositor

   Trina Schroer

   Article

   Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

   Insert

   Polo-like kinase 1 (PLK1 Human, BC01486)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   H538A/K540M

   Promoter

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

5. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

   Plasmid

   #121799

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOA-Leu-FvPPT1

   Plasmid

   #179923

   Purpose

   Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Insert

   FvPPT1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Promoter

   Yarrowia lipolytica EXP1

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2_2xNOP1gRNA-Cas9-CsA

   Plasmid

   #136140

   Purpose

   Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. deltaVP1 only

   Plasmid

   #166675

   Purpose

   Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121803

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EcM2.1.dtolC

    Bacterial Strain

    #64053

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Ampicillin

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. EcM2.1 dtolC bla::zeoR

    Bacterial Strain

    #64054

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Bleocin (zeocin)

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. MG1655 tnaA-

    Bacterial Strain

    #228513

    Purpose

    Indole-responsive Phage Infection Assay using LARP and T7 Phage with gp1 knock-out

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Bacterial Resistance

    None

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

14. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Promoter

    Available since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

    Plasmid

    #156438

    Purpose

    For transient expression of mouse CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse CTCF (Ctcf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti HsATP13A3 D498N

    Plasmid

    #195850

    Purpose

    Expresses D498N mutant Homo sapiens ATP13A3

    Depositor

    Veerle Baekelandt

    Article

    Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

    Insert

    ATPase 13A3 D498N (ATP13A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

    Plasmid

    #156448

    Purpose

    For transient expression of human CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human CTCF (CTCF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2214

    Plasmid

    #158417

    Purpose

    AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple265 (PCP2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple265

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple349

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-PKHD1-myc

    Plasmid

    #108407

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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