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pVoPo-03

Plasmid

#187838

Purpose

Backbone for inducible expression system in Fusobacterium nucleatum

Depositor

Jörg Vogel

Publication

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTRANS_231

Plasmid

#91120

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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EC64420

Plasmid

#209517

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDD56

Plasmid

#119883

Purpose

Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Publication

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_EYFP

Expression

Bacterial

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C3001

Plasmid

#91094

Purpose

Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMOD_A1910

Plasmid

#91041

Purpose

Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0502

Plasmid

#91012

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiEKAR2G1

Plasmid

#40177

Depositor

Olivier Pertz

Publication

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LD-B

Plasmid

#209861

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1

Plasmid

#163625

Purpose

Expresses DCLK1

Depositor

Kenneth Westover

Publication

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Promoter

sv40

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-IP-EGFP-ULK2

Plasmid

#38201

Depositor

Noboru Mizushima

Publication

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Publication

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Promoter

CAG

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Publication

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Gateway Cloning

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK067

Plasmid

#65174

Purpose

Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConR1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

Plasmid

#111727

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q54 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

Plasmid

#111746

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q42 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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