Addgene

We narrowed to 9,781 results for: otos

Showing: 2281 - 2310 of 9781 results

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pRS303:3mCherry

Plasmid

#37549

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

3mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pRS304:3mCherry

Plasmid

#37550

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

3mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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CTraf

Plasmid

#27808

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

Cerulean Traf (TANK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pET22b-EC12-129/138

Plasmid

#47926

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Use

Tags

6xHis and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199487

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199488

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199489

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199490

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199491

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199492

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199497

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199498

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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PRS_8-Jaws-tdTomato

Plasmid

#192593

Purpose

AAV-vector for optogenetic silencing of noradrenergic neurons

Depositor

Simon Wiegert

Article

Maheu et al bioRxiv 2025.03.26.644382

Insert

Jaws

Use

AAV

Tags

tdTomato-ER2

Expression

Mammalian

Mutation

Promoter

PRSX8

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF

Plasmid

#139564

Purpose

expresses SznF in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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ylqF-pET21a

Plasmid

#30133

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2008 Sep . 72(4):1363-70.

Insert

ylqF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
RCAS(A) cRax (CC#524)

Plasmid

#15194

Depositor

Connie Cepko

Article

Chen et al Development. 2002 Dec . 129(23):5363-75.

Insert

Rax (RAX2 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-CFP II (pMIC II)

Plasmid

#52109

Purpose

a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-ECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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mKikGR-C1

Plasmid

#54656

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

Type

Empty backbone

Use

Tags

mKikGR

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1_Lifeact-mCherry

Plasmid

#67302

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-DsRed FP (pMIDsRed II)

Plasmid

#52110

Purpose

a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-DsRed

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
SypHer

Plasmid

#48250

Purpose

pH sensor

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Use

Tags

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
ClpP-his

Plasmid

#22144

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

ClpP-His6

Use

Tags

his

Expression

Bacterial

Mutation

C-terminal His tag

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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meGFP-hTRAK1

Plasmid

#188664

Purpose

human TRAK1 with N-terminal meGFP tag

Depositor

Thomas Schwarz

Insert

OIP106 (TRAK1 Human)

Use

Tags

meGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAH2-intein/mCherry

Plasmid

#71405

Purpose

Expresses suppression tRNA and mCherry inserted with a Npu DnaE intein

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Npu DnaE Intein insertion in mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only