We narrowed to 2,722 results for: LRE

Showing: 1521 - 1540 of 2722 results

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-PM-TORCAR

Plasmid

#64930

Purpose

Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

Lyn-TORCAR (EIF4EBP1 Synthetic, Human)

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
PiggyBac EF1a-eGFP-Puro U6 MMP2 g1

Plasmid

#170824

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

Plasmid

#170822

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROD1-T2A-PuroR

Plasmid

#162338

Purpose

Lentiviral expression of NEUROD1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EF13E

Plasmid

#42942

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN- Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-4EBP1 F113A

Plasmid

#81122

Purpose

expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

4EBP1 F113A (Eif4ebp1 Mouse)

Expression

Mammalian

Mutation

F113A mutation

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-shRNA

Plasmid

#82697

Purpose

For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

RNAi

Promoter

hPGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NLS

Plasmid

#64931

Purpose

Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR-NLS (EIF4EBP1 Synthetic, Human)

Tags

Cerulean, Nuclear localization signal (NLS), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

STIM1-Venus173-C (STIM1 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA4 CoV-2 nsp1 3xFLAG

Plasmid

#176057

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2842C

Plasmid

#139324

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 R2842C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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canxTM-mEmerald (cytosolic)

Plasmid

#186962

Purpose

Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) and add ER signal…

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_L_MARV

Plasmid

#135473

Purpose

Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

L gene

Expression

Mammalian

Mutation

silent mutation (C to T) at position 7861; insert…

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_

Plasmid

#201174

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBGreen-FIRE-puro

Plasmid

#139195

Purpose

Luminescent reporter of ERK activity

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBG99

Use

Luciferase and Retroviral

Tags

Fra1 PEST domain fragment (FIRE) and nuclear loca…

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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