We narrowed to 8,654 results for: pcDNA3

1. pcDNA3.1-Rho*-BERKY3

   Plasmid

   #158220

   Purpose

   BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 19, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCDNA3-hTICAM1-VenusFlag

   Plasmid

   #197632

   Purpose

   Human TRIF protein expression in mammalian cells

   Depositor

   Haian Fu

   Insert

   TICAM1 (TICAM1 Human)
   

   Tags

   Venus-Flag

   Expression

   Mammalian

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3β-FLAG-CBP-LD-HA

   Plasmid

   #32906

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   C/EBP (Crebbp Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   L1435A/D1436A

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)GαoB-91-NLuc

   Plasmid

   #207152

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

   Plasmid

   #131033

   Purpose

   Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRA (Tcra Mouse)
   TCRB (Tcrb Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-VEGFR3-V5/HIS

   Plasmid

   #205618

   Purpose

   Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR3 receptor tyrosine kinase (FLT4 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   T28M, S455R- Please see depositor comment

   Promoter

   CMV

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Lyn-dPlcR

   Plasmid

   #181832

   Purpose

   Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR
   

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-FluoSTEP-AKAR

   Plasmid

   #181844

   Purpose

   Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-AKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 p53 S15A

    Plasmid

    #69004

    Purpose

    mammalian expression of human p53 S15A mutant.

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    S15A

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1_nV5-AMOT (p130)

    Plasmid

    #172997

    Purpose

    constitutive expression of V5-tagged AMOT (p130)

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOT (p130) (AMOT Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 3xFlag IFIT3

    Plasmid

    #53553

    Purpose

    mammalian expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-FLAG-SOX4

    Plasmid

    #110360

    Purpose

    Mammalian expression of FLAG-tagged SOX4

    Depositor

    Carlos Moreno

    Article

    Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

    Insert

    SOX4 (SOX4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacking 5' UTR

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HtrA2-FLAG

    Plasmid

    #15938

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 HA-SUMO2-Q90P

    Plasmid

    #36448

    Purpose

    Expresses HA-Sumo2Q90P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutated Q90P

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-sACE2(WT)-Fc(IgG1)

    Plasmid

    #145163

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-CHRNA7-mGFP

    Plasmid

    #62629

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge