We narrowed to 17,500 results for: puro

1. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-mNeon-PuroR [M1G]

   Plasmid

   #171800

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 puro CXCR4 siRNA-1

   Plasmid

   #12271

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   CXCR4 siRNA (CXCR4 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe(puro) mouse Egr2

   Plasmid

   #108097

   Purpose

   Retroviral vector for expression of mouse Egr2

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr2 (Egr2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

   Plasmid

   #176932

   Purpose

   Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-PuroR Xlone eGFP

   Plasmid

   #179837

   Purpose

   Doxycycline-inducible expression of eGFP

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN ; Donor plas…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniTol2 x EF1a_EKAREN5 + PGK-puro

   Plasmid

   #167817

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H231L

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-RLuc-T2A-PuroR

    Plasmid

    #102320

    Purpose

    expression of r-luciferase

    Depositor

    Rudolf Jaenisch

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe puro IkB alpha M

    Plasmid

    #12332

    Depositor

    Inder Verma

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pLVX-Puro-mRuby-Sec23A

    Plasmid

    #36158

    Depositor

    David Stephens

    Article

    Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Lentiviral

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-puro SHP2 E76A

    Plasmid

    #8331

    Depositor

    Ben Neel

    Article

    Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E76A (activated mutant)

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-HA-UBE3B-IRES-Puro

    Plasmid

    #107345

    Purpose

    Expresses HA tagged UBE3B in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Puro-TDP-43-A382T

    Plasmid

    #133756

    Purpose

    Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Changed alanine 382 to threonine

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLD-puro-Cc-TARDBP-A315T_VA

    Plasmid

    #141329

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-A315T (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Mutation

    A315T

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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