We narrowed to 18,191 results for: puro

Showing: 1521 - 1540 of 18191 results

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pPBT/EFS-Cas9-PuroTk

Plasmid

#170542

Purpose

Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

PuroTK

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Tags

pEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgRNA-CMV-puro

Plasmid

#169450

Purpose

backbone for sgRNA expression

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

sgRNA

Use

Lentiviral

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1

Plasmid

#114022

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE Tight

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Promoter

Ubc

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P

Plasmid

#202607

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3B-IRES-Puro

Plasmid

#107345

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-PGK-RAP1A-WT-puro

Plasmid

#156166

Purpose

Lentiviral vector for expression of RAP1A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW PRKN-P2A-PuroR

Plasmid

#239571

Purpose

Overexpression of human PARK2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

PRKN (PRKN Human)

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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OCT4-eGFP-PGK-Puro

Plasmid

#31937

Purpose

Oct4 targeting donor vector

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 A775_G776insV, G776C

Plasmid

#40979

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A775_G776insV, G776C

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
Lenti-guide-puro mCsnk2b - 1

Plasmid

#198505

Purpose

lentiviral stable expression of mCsnk2b gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCsnk2b (Csnk2b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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