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We narrowed to 18,387 results for: puro

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Showing: 1521 - 1540 of 18387 results

  1. pCDH-2xMARS-nGFP-puro

    Plasmid
    #205238
    Purpose
    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Lentiviral
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205238-plasmid-map-sequence-id-403865

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  3. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  5. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  8. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48226-plasmid-map-sequence-id-69514

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Showing: 1521 - 1540 of 18387 results