We narrowed to 2,896 results for: GEM;

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ZP43 Wnt8 3' orf1

Plasmid

#16899

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 3' orf1 (wnt8a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pTC151

Plasmid

#70015

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Expression

Plant

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTU2_2a

Plasmid

#73010

Purpose

Secondary module (BpiI) site in pTU2a backbone

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Secondary module + Golden Gate pTU2a BsmBI destination site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pde6d

Plasmid

#105464

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde6d (Pde6d Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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ZE19 wnt8 pBand3-5

Plasmid

#17053

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 pBand3-5 (wnt8a Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDS68

Plasmid

#48366

Purpose

GFP-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

GFP-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTU1-B-lacZ

Plasmid

#72936

Purpose

Level 1 Destination vector - Position B

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - B site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBW206J106-gfp

Plasmid

#78645

Purpose

pSB3K3 carrying J106-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J106-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW201J109-gfp

Plasmid

#78640

Purpose

pSB3K3 carrying J109-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW204J115-gfp

Plasmid

#78643

Purpose

pSB3K3 carrying J115-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz3

Plasmid

#74362

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

eashirt zinc finger family member 3 (Tshz3 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTU3-A-RFP

Plasmid

#72945

Purpose

Level 3 Destination vector - Assembly of 2 Level 2 modules - AB

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTU3-B-RFP

Plasmid

#72946

Purpose

Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - B site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pK812

Plasmid

#44622

Depositor

Robert Oshima

Article

Kulesh et al Mol Cell Biol. 1989 Apr;9(4):1553-65.

Insert

K8 (KRT8 Human)

Use

Cloning

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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ZE20 wnt8 pBand1-2

Plasmid

#17054

Depositor

Randall Moon

Article

Lekven et al Dev Cell. 2001 Jul;1(1):103-14. doi: 10.

Insert

wnt8 pBand1-2 (wnt8a Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRB7-6

Plasmid

#24700

Depositor

George Allen

Article

Allen et al Plant Cell. 1996 May . 8(5):899-913.

Insert

Tobacco Rb7 MAR (SAR)

Use

E. coli phagemid

Available Since

July 8, 2010

Availability

Academic Institutions and Nonprofits only
NSK162 CMV-TO-SMN2 Nluc - inactive (exon 6, 8)

Plasmid

#247275

Purpose

Expresses human SMN2 exons 6, 8 followed by inactive Nluc

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SMN2 exons 6,8-Nluc (SMN2 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Article

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

none

Promoter

SP6

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-FLAG-dCas9

Plasmid

#190130

Purpose

Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

FLAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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JGI_018363

Plasmid

#158859

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Gemmati_022_1075

Use

Synthetic Biology

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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DT039A

Plasmid

#159400

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23117 (weak) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT038A

Plasmid

#159399

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23106 (medium) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT040A

Plasmid

#159401

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

hybrid J23117-J23106 promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018343

Plasmid

#158849

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Gemmati_022_16911

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge