We narrowed to 1,917 results for: FRT

1. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

   Plasmid

   #90013

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-Dicer
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

   Plasmid

   #90015

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PTPRA-MAC

   Plasmid

   #139632

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA (PTPRA Human)
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-ARP8

   Plasmid

   #15361

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   actin-related protein 8 (ACTR8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

   Plasmid

   #169737

   Purpose

   Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-mCherry-attP(C31)-*BleoR

   Plasmid

   #183752

   Purpose

   phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PL652

   Plasmid

   #159456

   Purpose

   Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid

    #114038

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    MAD2 binding deficient MAD1 K541A/L543A

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-Adrm1-Flag

    Plasmid

    #19418

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

    Plasmid

    #90012

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-FLJ20309

    Plasmid

    #15360

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    FLJ20309 106-544residues (INO80D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

15. BirA-Myc_N_HNF1a

    Plasmid

    #167710

    Purpose

    Expresses HNF1a in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    HNF1a (HNF1A Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM296

    Plasmid

    #134180

    Purpose

    Mammalian expression of CENP-C and KNL2 CDK SA

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 HA-CCDC95

    Plasmid

    #19795

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    CCDC95 (INO80E Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

18. sgFFL_3x_siRNA

    Plasmid

    #59893

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has three siRNA-like targets (fully c…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

    Plasmid

    #215910

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332D, S1346D, S1616D, S169…

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215911

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge