We narrowed to 144,476 results for: ins

1. pDD285

   Plasmid

   #66826

   Purpose

   mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. HIV-1 integrase F185K/C280S in pET-15b

   Plasmid

   #61669

   Purpose

   HIV-1 integrase F185K/C280S in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   HIV-1 integrase F185K/C280S
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Mutation

   Promoter

   T7 promotor

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pSC101-GFPmut2-mScarlet-I TAG

   Plasmid

   #208188

   Purpose

   nonsense mistranslation, TAG stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TAG*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC101-GFPmut2-mScarlet-I TGA

   Plasmid

   #208189

   Purpose

   nonsense mistranslation, TGA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSC101-GFPmut2-mScarlet-I TGATC

   Plasmid

   #208190

   Purpose

   nonsense mistranslation, TGA stop variant 2

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7TCT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSC101-GFPmut2-mScarlet-I T7

   Plasmid

   #208191

   Purpose

   frameshift mistranslation, T7

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6TTTTTTT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSC101-GFPmut2-mScarlet-I A5

   Plasmid

   #208192

   Purpose

   frameshift mistranslation, A5

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC101-GFPmut2-mScarlet-I A4

   Plasmid

   #208193

   Purpose

   frameshift mistranslation, A4

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC101-GFPmut2-mScarlet-I A6T

   Plasmid

   #208194

   Purpose

   frameshift mistranslation, A6T

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAAAT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC101-GFPmut2-mScarlet-I C3T

    Plasmid

    #208195

    Purpose

    frameshift mistranslation, C3T

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6CCCT

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cmv d0 dPspCas13b-GGS-NYTHDF1

    Plasmid

    #119855

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAraH6HATT

    Plasmid

    #63901

    Purpose

    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Insert

    TT Fab
    

    Use

    Tags

    6xHis and HA tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-HxB.L

    Plasmid

    #65414

    Purpose

    contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    TNC large splice variant (TNC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP2
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCY 3010-02

    Plasmid

    #36210

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yeast-enhanced (yE) polylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-hphMX4

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV500 A-SREBP2

    Plasmid

    #33358

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Insert

    SREBP2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pUASP dsRed Rab27 WT

    Plasmid

    #44832

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab27 (Rab27 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pSNAPf-AP

    Plasmid

    #61830

    Purpose

    Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    SNAP-AP
    

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pTrc_AraC-I113-LOV

    Plasmid

    #206804

    Purpose

    Expresses AraC-I113-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTrc_AraC-S170-LOV

    Plasmid

    #206805

    Purpose

    Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge