We narrowed to 13,564 results for: cas9 genes

1. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIN-PGK-Cas9-V5-WPRE

   Plasmid

   #87876

   Purpose

   To produce lentiviral vector for Cas9 editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Human codon-optimized

   Promoter

   mouse PGK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXGFPC-5 Pxyl-dcas9 (Spy)

   Plasmid

   #133316

   Purpose

   expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

   Plasmid

   #149590

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIN-CMV-Cas9-V5-WPRE

   Plasmid

   #87904

   Purpose

   To produce lentiviral vector for gene editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   Cas9-V5
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

    Plasmid

    #149594

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN-PGK-Cas9-WPRE

    Plasmid

    #87886

    Purpose

    To produce Lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    hCas9
    

    Use

    Lentiviral

    Tags

    nls

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD2-dcas9-J23101-GFP

    Plasmid

    #122260

    Purpose

    Constitutive dCas9 and GFP expression plasmid

    Depositor

    Chueh Loo Poh

    Article

    Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-H1-sgGFP1-7SK-sgCas9

    Plasmid

    #87908

    Purpose

    multiple sgRNA

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgCas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

    Plasmid

    #141052

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK33/intein-Cas9_S219-3XFLAG

    Plasmid

    #133782

    Purpose

    Expresses human codon-optimized intein-Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Human codon-optimized intein-Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-Dpi2Cas9-puro

    Plasmid

    #232113

    Purpose

    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-Slu1Cas9-puro

    Plasmid

    #232111

    Purpose

    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-Slu2Cas9-puro

    Plasmid

    #232112

    Purpose

    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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