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We narrowed to 4,215 results for: bre

Showing: 3901 - 3950 of 4215 results
  1. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. hGFAP-GFP

    Plasmid
    #40592
    Depositor
    Insert
    GFP-S65T
    Use
    Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Bacterial and Mammalian
    Mutation
    Construct contains a mutated, humanized hGFP(S65T…
    Promoter
    hGFAP promoter
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
  3. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pEND-303_Cacnes_tetR

    Plasmid
    #225618
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
    Depositor
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pEND-304_Cacnes_tetR

    Plasmid
    #225619
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry
    Depositor
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pEND-375_Cacnes_phlF

    Plasmid
    #225620
    Purpose
    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.
    Depositor
    Insert
    PhlF
    Use
    Synthetic Biology
    Tags
    6xHis and mCherry-V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. Rlucrarr3-Flash1

    Plasmid
    #74129
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. Rlucrarr3-Flash4

    Plasmid
    #74132
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. Rlucrarr3-Flash3

    Plasmid
    #74131
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #217896
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag
    Depositor
    Inserts
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. pUC19-B9M9X8

    Plasmid
    #103072
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Insert
    B9M9X8(Cas9 coding gene from Acidovorax ebreus (strain TPSY))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pEND-350_Cacnes_ori

    Plasmid
    #225605
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid
    #222884
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Insert
    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid
    #222883
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Insert
    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid
    #222891
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Insert
    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid
    #222886
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Insert
    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid
    #222888
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Insert
    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid
    #222887
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Insert
    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid
    #222890
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Insert
    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 3901 - 3950 of 4215 results