We narrowed to 15,064 results for: pET

1. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-11a (GB1 WT - A34F/V39L)

   Plasmid

   #136610

   Purpose

   Expresses GB1 WT double mutant (A34F/V39L)

   Depositor

   Roberto Chica

   Article

   Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

   Insert

   GB1 WT double mutant (A34F/V39L)
   

   Use

   Tags

   6x-Histag

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: A34F - V39L

   Promoter

   T7 promoter

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-Synapsin-Ypet-3XNLS-WPRE

   Plasmid

   #159950

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   Ypet-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-Synapsin-Ypet-optoJNKi-WPRE

   Plasmid

   #159967

   Purpose

   optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   Ypet-optoJNKi
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-45b-HOXA13(+7A)-IDR-mCherry

   Plasmid

   #145285

   Purpose

   Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13(+7A)-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-45b-HOXA13(WT)-IDR-mCherry

   Plasmid

   #145284

   Purpose

   Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13(WT)-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15-His-∆NGlpG(C104A/M247C)

   Plasmid

   #155133

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C104A/M247C, insert codes for GlpG residues 87-276

   Promoter

   T7

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Avi-Coh (E154C)-HIS

   Plasmid

   #153447

   Purpose

   E. coli expression of Rc. CohE construct with E154C mutation.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Use

   Tags

   6xHis and Avi-tag

   Expression

   Bacterial

   Mutation

   E154C mutation on CohE

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-SznF H407A H409A H448A

    Plasmid

    #139566

    Purpose

    expressed SznF H407A H409A H448A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF H407A H409A H448A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET51b(+)_EGFP-LAMA-S98 (for p24)

    Plasmid

    #130717

    Purpose

    Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-LAMA-S98
    

    Use

    Tags

    Hisx10 and StrepTag

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET151-GST-PP-RPN13 (19-132) K97E

    Plasmid

    #125825

    Purpose

    Expresses GST-RPN13 (19-132) K97E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 97 to glutamate

    Promoter

    T7

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET151-GST-PP-RPN13 (19-132) K83A

    Plasmid

    #125829

    Purpose

    Expresses GST-RPN13 (19-132) K83A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 83 to alanine

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET151-GST-PP-RPN13 (19-132) K97A

    Plasmid

    #125823

    Purpose

    Expresses GST-RPN13 (19-132) K97A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 97 to alanine

    Promoter

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET151-GST-PP-RPN13 (19-132) K97D

    Plasmid

    #125824

    Purpose

    Expresses GST-RPN13 (19-132) K97D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 97 to aspartate

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET151-GST-PP-RPN13 (19-132) R104A

    Plasmid

    #125826

    Purpose

    Expresses GST-RPN13 (19-132) R104A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to alanine

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-GST-PP-RPN13 (19-132) R104D

    Plasmid

    #125827

    Purpose

    Expresses GST-RPN13 (19-132) R104D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to aspartate

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET151-GST-PP-RPN13 (19-132) R104E

    Plasmid

    #125828

    Purpose

    Expresses GST-RPN13 (19-132) R104E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to glutamate

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pet28a His6-HSPB7 (zebrafish) C49S

    Plasmid

    #110071

    Purpose

    Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 49 to Serine

    Promoter

    T7

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET11a asyn Q79N (TAC-->TAT)

    Plasmid

    #105733

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79N

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pet151D-TOPO-BROX 1-411; K141E

    Plasmid

    #89865

    Purpose

    Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Use

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated lysine 141 to glutamic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pet151D-TOPO-BROX 1-411; L212D

    Plasmid

    #89864

    Purpose

    Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Use

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated leucine 212 to aspartic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET151-GFP-PP-RPN2 (918-953)

    Plasmid

    #73743

    Purpose

    Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pET151-GFP-PP-RPN2 (932-953)

    Plasmid

    #73745

    Purpose

    Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pET151-GFP-PP-RPN2 (918-947)

    Plasmid

    #73747

    Purpose

    Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-947 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pET151-GFP-PP-RPN2 (797-953)

    Plasmid

    #74228

    Purpose

    [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

28. pET151-Vps4(101-437) L151D

    Plasmid

    #87736

    Purpose

    Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Leu 151 to Asp

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET151-hUCH37 (ISF1)(M148D/F149D)

    Plasmid

    #71388

    Purpose

    Expression of hUCH37 (M148D/F149D) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
    

    Use

    Tags

    HIS-TEV sequence

    Expression

    Bacterial

    Mutation

    changed methionine 148 to aspartate, phenylalanin…

    Promoter

    T7

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pET151-hUCH37 (ISF1)(M148A/F149A)

    Plasmid

    #61933

    Purpose

    Expression of hUCH37 (M148A/F149A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Methionine 148 and Phenylalanine 149 were mutated…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

34. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available Since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-22HT-MSI1(7-192)Q30D

    Plasmid

    #60346

    Purpose

    Q30D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    Q30D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Dec. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pET-22HT-MSI1(7-192)R53E

    Plasmid

    #60352

    Purpose

    R53E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pET-22HT-MSI1(7-192)G64A

    Plasmid

    #60351

    Purpose

    G64A mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G64A; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pET-22HT-MSI1(7-192)G35D

    Plasmid

    #60347

    Purpose

    G35D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pET-22HT-MSI1(7-192)G35E

    Plasmid

    #60348

    Purpose

    G35E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

42. pET-22HT-MSI1(7-192)H83F

    Plasmid

    #60349

    Purpose

    H83F mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83F; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET15b/GgVcl 1-851 A50I mutation

    Plasmid

    #46174

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 A50I mutation (talin binding domain mut…

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET15b/GgVcl 1-258 A50I mutation

    Plasmid

    #46175

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1) A50I mutation; talin binding defic…

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid

    #39337

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

47. pET-21a(+)-DR RecR(K23A/R27A)

    Plasmid

    #12619

    Depositor

    SeWon Suh

    Article

    Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

    Insert

    Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysine 23 to Alanine, and Arginine 27 to …

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. p141 pETF-GST RanBP10 G308R

    Plasmid

    #11094

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G308R

    Promoter

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Pet28a His6-HSPB7 (zebrafish) C117S

    Plasmid

    #110072

    Purpose

    Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 117 to Serine

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge