We narrowed to 1,976 results for: 974

1. plenti-UbcP-FLAG-YWAA-pGK-HYG

   Plasmid

   #107375

   Purpose

   Lentiviral expression of FLAG-YWAA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CRBN-Y383A/W385A

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1_sh_ex13

   Plasmid

   #35165

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLKO-tet-puro-sh-ex16

   Plasmid

   #35167

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMXs_FLAG-MCART1W283A

   Plasmid

   #138409

   Purpose

   Express FLAG-tagged MCART1W283A in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   SLC25A51 (SLC25A51 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells, W2…

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-tet-puro-sh-ex16b

   Plasmid

   #35168

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. psiCHECK2-CCNA2 LONG 3'UTR

   Plasmid

   #29472

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   CCNA2 LONG 3'UTR (CCNA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pD10AH840AhCas9 (GB1041)

   Plasmid

   #68207

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-BFP-Mito

   Plasmid

   #237398

   Purpose

   Lentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells

   Depositor

   James Hurley

   Article

   Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

   Insert

   COX8A (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Aug. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Use

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MZB035

    Plasmid

    #217834

    Purpose

    Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Inserts

    AP3D1 (AP3D1 Human)
    AP3S1 (AP3S1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Removed residues 618-1153 from AP3D1

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. TadA-N46L-NG-CBE

    Plasmid

    #227419

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-N46L-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. YEE-NG-CBE

    Plasmid

    #227418

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSD090A

    Plasmid

    #218132

    Purpose

    Hybrid circuit displaying mmCRBN bait and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    Crbn (Crbn Mouse)
    luxAB
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSD094B

    Plasmid

    #218131

    Purpose

    Hybrid circuit displaying mmCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    Crbn (Crbn Mouse)
    M13 pIII
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

    Plasmid

    #212113

    Purpose

    Expression of GABARAP split tandem protein with mCherry

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mCherry-GABARAP (GABARAP Discosoma sp., Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of upstream GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

    Plasmid

    #212109

    Purpose

    Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPR114-DuET

    Plasmid

    #213258

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR114 (ADGRG5 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGADT7-ε

    Plasmid

    #197261

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitutions in codons 905, 1129 and 1137

    Promoter

    ADH1

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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