We narrowed to 14,072 results for: CRISPR-Cas9

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pCMV_EE-nCas9

Plasmid

#163541

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1003K

Plasmid

#207371

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1003K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A848K

Plasmid

#207369

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A848K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A661R

Plasmid

#207367

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-Q661R

Plasmid

#207366

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-Q661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, K526E, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-N515Y

Plasmid

#207364

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-N515Y

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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HypaR-SpCas9

Plasmid

#126757

Purpose

Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Usp5

Plasmid

#174873

Purpose

CRISPR vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Usp5 (Usp5 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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St1Cas9_LMD-9_sgRNA_pUC19

Plasmid

#110627

Purpose

U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Insert

Empty St1Cas9 LMD-9 sgRNA backbone

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Mammalian

Promoter

hU6

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pICH47811-pAtAct2::Cas9-nls::tSlATPase

Plasmid

#160221

Purpose

Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtACT2::Cas9-nls::tSlATPase

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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