We narrowed to 17,501 results for: puro

1. pTRIP-CMV-puro-2A-BLM

   Plasmid

   #127641

   Purpose

   Over-expression of human BLM

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   BLM (BLM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTet-O-NEUROG1-T2A-PuroR

   Plasmid

   #162339

   Purpose

   Lentiviral expression of NEUROG1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROG1 (NEUROG1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/Puro-CAG-JEDI-2P-Kv

   Plasmid

   #179462

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P-Kv
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCRISPRv2-PURO-LYSET-gRNA1

   Plasmid

   #210489

   Purpose

   CRISPR pooled KO of human LYSET

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-gRNA1 (LYSET Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. plentiCRISPRv2-PURO-LYSET-gRNA2

   Plasmid

   #210490

   Purpose

   CRISPR pooled KO of human LYSET

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-gRNA2 (LYSET Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro-NLS-LSSmKate2

   Plasmid

   #34586

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS-LSS-mKate2
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Promoter

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe(puro) mouse Egr3

   Plasmid

   #108102

   Purpose

   Retroviral vector for expression of mouse Egr3

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr3 (Egr3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-puro/Flag PD-L1 N200Q

    Plasmid

    #121451

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N200Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-FlaviA-GFP-puro

    Plasmid

    #140088

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid

    #208042

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid

    #193764

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔCy-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-ND-Cdt1-mCherry-Puro

    Plasmid

    #193766

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    ND-Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-Puro-Flag-IKBKE

    Plasmid

    #15292

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pLVX-Puro-mGSDME F2A

    Plasmid

    #154878

    Purpose

    Expresses mGSDME F2A in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changes Phenylalanine 2 to Alanine

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-UBE3B-HA-IRES-Puro

    Plasmid

    #107346

    Purpose

    Expresses HA tagged UBE3B in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHDR_REC8-T2A-mGreenLantern-puroTK

    Plasmid

    #222521

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to REC8.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPOTv6-puro-3Ty:mNG:3Ty

    Plasmid

    #179798

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mNG
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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