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Showing: 141 - 160 of 397 results

  1. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  2. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  3. pJK508

    Plasmid
    #71598
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71598-plasmid-map-sequence-id-131672

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  4. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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  5. pDNR-hSox2

    Plasmid
    #49389
    Purpose
    donor vector for cloning human Sox2 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Expression
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    none
    Available since
    Nov. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pJK600

    Plasmid
    #71605
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71605-plasmid-map-sequence-id-131682

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  10. pDNR-hAkt1

    Plasmid
    #49387
    Purpose
    donor vector for cloning human Akt1 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Expression
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    none
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

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  20. pJK382

    Plasmid
    #71574
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to asparagine; silent mutat…
    Promoter
    recA
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71574-plasmid-map-sequence-id-130942

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Showing: 141 - 160 of 397 results