We narrowed to 935 results for: lentiviral vector plasmid

1. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

   Plasmid

   #214471

   Purpose

   Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S14D S25D S32D T261D

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

   Plasmid

   #154987

   Purpose

   Lentiviral vector expressing Human ACE2 and TMPRSS2

   Depositor

   Sonja Best

   Insert

   ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

   Plasmid

   #154983

   Purpose

   Lentiviral vector expressing Human ACE2 and TMPRSS2

   Depositor

   Sonja Best

   Insert

   ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177435

   Purpose

   To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

   Depositor

   David Andrews

   Insert

   Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-L3US2-RFP-HDAC3

   Plasmid

   #83966

   Purpose

   Lentiviral CRISPR HDAC3 dual gRNA targeting vector

   Depositor

   Eleanor Chen

   Insert

   HDAC3 dual gRNAs (HDAC3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK DNM1L-V5 neo

   Plasmid

   #220363

   Purpose

   Lentiviral expression vector for an inducible V5-tagged DNM1L construct

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLgw ELK1Y158A-V5-EcoDam

   Plasmid

   #98609

   Purpose

   Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Insert

   ELK1Y158A (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLgw EcoDam-V5-ELK1Y158A

   Plasmid

   #98608

   Purpose

   Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Insert

   ELK1Y158A (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTJK477

   Plasmid

   #138005

   Purpose

   3xMYC-SIX2 expression. Vector also expresses GFP under control of hUBC promoter

   Depositor

   Tami Kingsbury

   Insert

   3xMYC tagged SIX2 (SIX2 Human)
   

   Use

   Lentiviral

   Tags

   3xMYC

   Expression

   Mutation

   None

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTJK421

    Plasmid

    #138007

    Purpose

    BirA*-SIX2 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    BirA* tagged SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    BirA*

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCheck2 Dest (R1-R2) #BGV129

    Plasmid

    #48955

    Purpose

    Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Luciferase; Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS-FuG-B

    Plasmid

    #67523

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

    Insert

    Fusion Glycoprotein type B
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. phH1-gRNA

    Plasmid

    #53186

    Purpose

    Expresses the S. pyogenes sgRNA from the H1 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human H1

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human 7SK

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScalps hygro

    Plasmid

    #184785

    Purpose

    Empty Lentiviral vector backbone

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTJK438

    Plasmid

    #138004

    Purpose

    3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    3xMYC tagged SIX1 (SIX1 Human)
    

    Use

    Lentiviral

    Tags

    3xMYC

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCD/NL-BH∆1

    Plasmid

    #41791

    Purpose

    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    HIV-1 Gag/Pol and RRE sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBEG R2-L3 #BGV193

    Plasmid

    #48952

    Purpose

    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pBEG R3-L4 #BGV194

    Plasmid

    #48954

    Purpose

    For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

25. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCG-Hc∆18

    Plasmid

    #84817

    Purpose

    Measles virus hemagglutinin expressing plasmid

    Depositor

    Jakob Reiser

    Article

    Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-L4-UBI-L1R

    Plasmid

    #45959

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Methods Mol Biol. 2011;737:183-209. doi:

    Insert

    human UBI promoter (UBC Synthetic, Human)
    

    Use

    Pentr plasmid

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUltra-H2B-mTagBFP2

    Plasmid

    #161791

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    EGFP and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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