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We narrowed to 2,024 results for: ARP

Showing: 141 - 160 of 2024 results

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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T7-His-Arp11(1–417)

Plasmid

#51400

Purpose

expression of full-length mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

Plasmid

#228508

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I5CHO (#1)

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Expression

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_ARPC1B:1-372

Plasmid

#210905

Purpose

Insect expression of full-length human ARPC1B. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_ARPC1B:1-372

Plasmid

#211195

Purpose

Mammalian expression of full-length human ARPC1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ARPC1A:1-370

Plasmid

#211267

Purpose

Mammalian expression of full-length human ARPC1A. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1A:M1-M370 (ARPC1A Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ARPC1B:1-372

Plasmid

#211268

Purpose

Mammalian expression of full-length human ARPC1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-del.p119K120S–eGFP

Plasmid

#211554

Purpose

A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

eGFP

Expression

Mammalian

Mutation

deletion of amino acids 119K 120S

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLarp_N

Plasmid

#146982

Purpose

Insect Expression of DmLarp

Depositor

Elisa Izaurralde

Insert

DmLarp (larp Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutation N110T, P173L compared to …

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Use

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP

Plasmid

#185516

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

Replaces Q to P in GARQYNLI

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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