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Plasmid#87135PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorUseTagsExpressionBacterialMutationD196A, L197RPromoterwaaHp from E. coli as part of operon with ald* an…Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only
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pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
UseTagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 (CD)
Plasmid#89736PurposeExpresses the CD-domain truncation to mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 CD mutant
Plasmid#89896PurposeExpresses mouse TET2 CD mutant in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
∆cosσε ∆HG
Bacterial Strain#197132PurposeDeficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.DepositorBacterial ResistanceHygromycinAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
V. natriegens NC7
Bacterial Strain#215356PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
V. natriegens NC1
Bacterial Strain#215355PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_AQUAR
Plasmid#204239PurposeBacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_AQUAR
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK137
Plasmid#73313PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6 and c-mycExpressionYeastMutationsynthetic genePromoter5'-AOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK108
Plasmid#73312PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)DepositorInsertdelta-12 fatty acid acetylenase
UseTagsExpressionBacterialMutationsynthetic genePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
DH5alpha::DddI
Bacterial Strain#179631PurposeFor expression of toxic DddADepositorBacterial ResistanceGentamicinAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_CHLP8
Plasmid#204242PurposeBacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_CHLP8
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL32 (in cHNL135)
Plasmid#24387DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHC_YEAST
Plasmid#204232PurposeBacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC_YEAST (MDH2 Budding Yeast)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHC1_ARATH
Plasmid#204237PurposeBacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC1_ARATH (AT1G04410 Mustard Weed)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDH_PFALCI
Plasmid#204235PurposeBacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only