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Showing: 141 - 160 of 605 results

  2. ULK1-Halo HRD

    Plasmid
    #207548
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    207548-plasmid-map-sequence-id-430489

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  15. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  17. pcDNA3 RL3Am

    Plasmid
    #109364
    Purpose
    Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag
    Depositor
    Robert Lucas
    Article
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Insert
    Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Human, Branchiostoma belcheri)
    Use
    Tags
    1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    109364-plasmid-map-sequence-id-210252

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  18. 3xMS-TR-100-PURO HRD

    Plasmid
    #207582
    Purpose
    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    Use
    Tags
    3xMS2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207582-plasmid-map-sequence-id-429467

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  19. mCherry-Dyskerin

    Plasmid
    #207577
    Purpose
    N-terminal mCherry-tag using SalI/EcoRI
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207577-plasmid-map-sequence-id-429490

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  20. Halo-TCAB1 HRD

    Plasmid
    #207535
    Purpose
    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207535-plasmid-map-sequence-id-430972

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Showing: 141 - 160 of 605 results