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Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
UseTags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3)-R3-pRARE2
Bacterial Strain#26242PurposePhage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.DepositorBacterial ResistanceChloramphenicolAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3) ΔserC
Bacterial Strain#197656PurposeDerivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.DepositorBacterial ResistanceNoneAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB
Bacterial Strain#176582PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 GamS
Bacterial Strain#176584PurposeE. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD GamS
Bacterial Strain#176586PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
E. coli BL21 (DE3) ΔEntD
Bacterial Strain#192874PurposeDeletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.DepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB GamS
Bacterial Strain#176585PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-40)
Plasmid#71876PurposeExpresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q-R388G
Plasmid#184251PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCRW1
Plasmid#122462PurposeHuman NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cellsDepositorUseTagsHis TagExpressionBacterialMutationPromoterT7lac promoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PermaPhos Kit
Plasmid Kit#1000000226PurposeThis kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression systemDepositorAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain
Bacterial Strain#99247PurposeLacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strainDepositorBacterial ResistanceTetracyclineAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rinehart & Söll Phosphoprotein Synthesis Kit
Plasmid Kit#1000000020PurposeAllows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate SepâtRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).DepositorAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlkB
Plasmid#228218PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
UseTagsHis tagExpressionBacterialMutationcodon optimizedPromoterT7Available sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only