We narrowed to 5,563 results for: cycs

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pACYC-TtCas6-4xcrRNA4.5

Plasmid

#127764

Purpose

For expression of T. thermophilus Csm complex in E. coli (crRNA)

Depositor

Jennifer Doudna

Article

Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

Inserts

Synthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available Since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
Cyclin E-pBI-EGFP

Plasmid

#16654

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

Cyclin E (CCNE1 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE puro cyclinD1 HA

Plasmid

#9050

Depositor

William Hahn

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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MultiMate-Cell-cycle

Plasmid

#206280

Purpose

Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B iRFP713, mAG-hGeminin, mKO2-hCdt1

Use

Recombinant baculovirus production

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTO135_pENO1-CaCas9-tCyc1

Plasmid

#171103

Purpose

Expresses Cas9 in Candida auris

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

CaCas9

Expression

Yeast

Promoter

ENO1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12deltaRS/Cyclin K

Plasmid

#231744

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3/CyclinB1-GFP (28)

Plasmid

#39871

Depositor

Jonathon Pines

Article

Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

Insert

CyclinB1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Mutation

V5A

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA cyclin D1 HA T286A

Plasmid

#11182

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 T286A (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

T286A. Mutant that cannot be degraded via the u…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-HA

Plasmid

#222711

Purpose

Expression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCeru-N1 cyclin A (1557)

Plasmid

#61842

Purpose

mammalian expression of cyclin A with cerulean fusion

Depositor

Jonathon Pines

Insert

cyclin A (CCNA2 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse cyclin D2

Plasmid

#86791

Purpose

bacterial expression of mouse cyclin D2

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D2 (Ccnd2 Mouse)

Expression

Bacterial

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGal CycK (P#844)

Plasmid

#14664

Depositor

Matija Peterlin

Article

Lin et al J Biol Chem. 2002 May 10. 277(19):16873-

Insert

CycK (CCNK Human)

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pACYC-PLlambda-alkSm4-3

Plasmid

#44383

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkSm4-3

Use

Synthetic Biology

Mutation

Q410I, S470T, K546E, E775D

Promoter

PLlambda

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pBIG1e-CDK12/Cyclin K

Plasmid

#231730

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK13/Cyclin K

Plasmid

#231731

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK13 (CDK13 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pVenus-N1 Cyclin B1 (1148)

Plasmid

#26062

Depositor

Jonathon Pines

Insert

Cyclin B1 (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only
cyclin D1-HA-pBabe-Hygro

Plasmid

#22703

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+CyclinB1-GFP

Plasmid

#128426

Purpose

Contains insert allowing in-vivo visualization of CyclinB1 protein (G2/M)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

CyclinB1-GFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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