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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 141 - 160 of 4839 results

  10. pCRISPR-S12

    Plasmid
    #84031
    Purpose
    To episomally express codon optimized Cas9 and chimeric guide RNA
    Depositor
    Sen Wu
    Publication
    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hSpCas9
    eGFP
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    CMV and CMV (downstream of F2A self-cleaving pept…
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    84031-plasmid-map-sequence-id-192362

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  11. pJH136

    Plasmid
    #47554
    Purpose
    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.
    Depositor
    Ronald Davis
    Publication
    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10
    Insert
    URA3
    Use
    Pcr template
    Promoter
    Native URA3 promoter, -243 to -1.
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    47554-plasmid-map-sequence-id-67878

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  19. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP160
    Mutation
    D10A;H840A nuclease-deficient
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    48221-plasmid-map-sequence-id-69506

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Showing: 141 - 160 of 4839 results