Addgene

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBpaRS3tRNA

Plasmid

#197102

Purpose

To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

Insert

TyrRS variant, amber suppressor tRNA

Use

Tags

Expression

Bacterial

Mutation

Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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SBC015866

Plasmid

#226280

Purpose

Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

Depositor

Rainer Breitling

Article

Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

Inserts

4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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StrKDEL-IRES-ManII-mScarlet-i

Plasmid

#117274

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

partial sequence of mannosidase II (Man2a1 Mouse)
Streptavidin-KDEL

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV and IRES

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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SBC015869

Plasmid

#226281

Purpose

Expresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

Depositor

Rainer Breitling

Article

Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

Inserts

4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAcBac1-Mb-Fluoro-Phe D6

Plasmid

#197577

Purpose

Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

Inserts

Mb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)

Use

Tags

Nuclear export sequence + FLAG

Expression

Mammalian

Mutation

N311A, C313M, V366G, W382T

Promoter

CMV and U6/H1

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-Fluoro-Phe B5

Plasmid

#197576

Purpose

Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

Inserts

Mb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)

Use

Tags

Nuclear export sequence + FLAG

Expression

Mammalian

Mutation

N311G, C313A

Promoter

CMV and U6/H1

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES-mannosidase II-mTagBFP2

Plasmid

#165460

Purpose

Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

Partial ManII-mTag2BFP fusion (Man2a1 Mouse)

Use

Tags

Str-KDEL and mTag2BFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Target Accelerator Genetic Pathway Reference Set

Plasmid Kit

#1000000105

Purpose

Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes

Depositor

Jesse Boehm

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only
pGLU_66

Plasmid

#225769

Purpose

This is the native promoter from the catP chloramphenicol resistance cassette.

Depositor

Nili Ostrov

Insert

PcatP_[abr]

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mGFP

Plasmid

#190027

Purpose

Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

Depositor

Andrew Tutt

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_3xFlag

Plasmid

#190028

Purpose

Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag

Depositor

Andrew Tutt

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-L3L2

Plasmid

#114019

Purpose

Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

Depositor

Christopher Grefen

Insert

ccdB gene, Chloramphenicol resistance gene

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-L1L4

Plasmid

#114018

Purpose

Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

Depositor

Christopher Grefen

Insert

ccdB gene, Chloramphenicol resistance gene

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-L1L2

Plasmid

#114017

Purpose

Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

Depositor

Christopher Grefen

Insert

ccdB gene, Chloramphenicol resistance gene

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBD026

Plasmid

#201309

Purpose

AvrSr27-2 as Golden Gate compatible MoClo CDS part

Depositor

Benjamin Schwessinger

Insert

AvrSr27-2

Use

Tags

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBD025

Plasmid

#201308

Purpose

AvrSr27-1 as Golden Gate compatible MoClo CDS part

Depositor

Benjamin Schwessinger

Insert

AvrSr27-1

Use

Tags

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSB4A3-PBAD-phzO

Plasmid

#165619

Purpose

Arabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazine

Depositor

Martin Buck

Insert

phzO

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PROM_3

Plasmid

#201296

Purpose

D3: Promoter of TraesCS3A02G510700 as Golden Gate compatible MoClo PROM part

Depositor

Benjamin Schwessinger

Insert

D3: Promoter of TraesCS3A02G510700

Use

Tags

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBD027

Plasmid

#201310

Purpose

avrSr27-3 as Golden Gate compatible MoClo CDS part (no recognition by Sr27)

Depositor

Benjamin Schwessinger

Insert

avrSr27-3

Use

Tags

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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