We narrowed to 5,193 results for: FAS

1. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

   Plasmid

   #202554

   Purpose

   Synaptotagmin-1 sgRNA1 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHybr_r2a>Fab-srt-his

   Plasmid

   #124810

   Purpose

   HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

   Depositor

   Martijn Verdoes, Ferenc Scheeren

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   rat IgG2a Hinge-G4S-Sortag-Histag
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial

   Mutation

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBlue-hFX-HA(NcoI/EcoRI)

   Plasmid

   #15405

   Depositor

   Michael Ristow

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1 and HA

   Expression

   Bacterial

   Mutation

   insert can be released by digestion with REs NcoI…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-basic-hFX-promotor(2kb, distal)

   Plasmid

   #14981

   Depositor

   Michael Ristow

   Insert

   human frataxin promoter fragment (FXN Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   includes distal fragment of human frataxin promot…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. FXN_Halo_N_allele

   Plasmid

   #178141

   Purpose

   Donor vector for endogenous tagging of human FXN at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (FXN Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBlue-hFX-HA(NcoI/EcoRI)

   Plasmid

   #15405

   Depositor

   Michael Ristow

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1 and HA

   Expression

   Bacterial

   Mutation

   insert can be released by digestion with REs NcoI…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-basic-hFX-promotor(2kb, distal)

   Plasmid

   #14981

   Depositor

   Michael Ristow

   Insert

   human frataxin promoter fragment (FXN Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   includes distal fragment of human frataxin promot…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. FXN_Halo_N_allele

   Plasmid

   #178141

   Purpose

   Donor vector for endogenous tagging of human FXN at the N-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (FXN Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p(bhsp68-Cas9)

    Plasmid

    #65959

    Purpose

    Tribolium basal heat shock promoter driving Cas9

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    nuclear localization sequence

    Expression

    Insect

    Mutation

    Promoter

    bhsp

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-basic-hFX-promotor(2,5kb)

    Plasmid

    #14980

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGL3-basic-hFX-promotor(1,3kb)

    Plasmid

    #14979

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-basic-hFX-promotor(1kb)

    Plasmid

    #14978

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pBlue-hFX(I154F)(AA88)-HA

    Plasmid

    #15403

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1, HA, and HIS

    Expression

    Bacterial

    Mutation

    contains cDNA coding sequence for amino acids 88-…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET28(a)-hFX(I154F)-HA

    Plasmid

    #14992

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1, HA, and HIS

    Expression

    Bacterial

    Mutation

    contains cDNA coding sequence for amino acids 88-…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. p(bhsp68-Cas9)

    Plasmid

    #65959

    Purpose

    Tribolium basal heat shock promoter driving Cas9

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    nuclear localization sequence

    Expression

    Insect

    Mutation

    Promoter

    bhsp

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-basic-hFX-promotor(2,5kb)

    Plasmid

    #14980

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pGL3-basic-hFX-promotor(1,3kb)

    Plasmid

    #14979

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only