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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Publication

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Publication

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Cdk4-HA

Plasmid

#1876

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Cdk4-HA

Plasmid

#1876

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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413 pSG5L HA RB

Plasmid

#10720

Depositor

William Sellers

Publication

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (RB1 Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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413 pSG5L HA RB

Plasmid

#10720

Depositor

William Sellers

Publication

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (RB1 Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Publication

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Publication

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Tags

V5

Expression

Mammalian

Promoter

TRE3G

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TetO-Ascl1-puro

Plasmid

#97329

Purpose

Generating lentiviruses to express Ascl1 and puromycin resistance

Depositor

Marius Wernig

Publication

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Promoter

TET-ON

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)

Viral prep

#83898-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Promoter

mDlx

Tags

mCherry

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Publication

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Publication

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pMXs-DsRed Express

Plasmid

#22724

Depositor

Shinya Yamanaka

Publication

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

a variant of Discosoma sp. red fluorescent protein

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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