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Showing: 1581 - 1600 of 6243 results

  1. PB-CAG::Nucbow

    Plasmid
    #158996
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158996-plasmid-map-sequence-id-311805

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  3. Tol2-CAG::Nucbow

    Plasmid
    #158992
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158992-plasmid-map-sequence-id-311797

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  6. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid
    #157732
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nprot-HF_GC3opt (N SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157732-plasmid-map-sequence-id-308146

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  7. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  8. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid
    #157701
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157701-plasmid-map-sequence-id-307865

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  9. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  11. pVITRO1-dV-IgG1/κ

    Plasmid
    #52213
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52213-plasmid-map-sequence-id-322304

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  12. pVITRO1-dV-IgG1/λ

    Plasmid
    #52214
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52214-plasmid-map-sequence-id-432867

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  15. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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Showing: 1581 - 1600 of 6243 results