We narrowed to 1,804 results for: FAST

1. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClCry4 Y319D

   Plasmid

   #160649

   Purpose

   Expression of protein for various experiments including finding interaction partners

   Depositor

   Joseph Takahashi

   Article

   Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

   Insert

   Clcry4 PHR Y319D
   

   Tags

   Hexa his

   Expression

   Bacterial

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTP288

   Plasmid

   #113460

   Purpose

   human Flag tagged Nbs1 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

   Insert

   Nbs1 (NBN Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP284

   Plasmid

   #113312

   Purpose

   human Nbs1 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

   Insert

   Nbs1 (NBN Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ULK1-Halo HRD

   Plasmid

   #207548

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Halo-TCAB1 HRD

   Plasmid

   #207535

   Purpose

   Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p130Cas

   Plasmid

   #186131

   Purpose

   For expression of recombinant p130Cas in SF9 cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Tags

   His6

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB-Flag-MyoD-E12

   Plasmid

   #25990

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD-E12 Heterodimer Fusion (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Available Since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-GTE-COI1

    Plasmid

    #29516

    Depositor

    Ning Zheng

    Article

    Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

    Insert

    COI1 (COI1 Mustard Weed)
    

    Tags

    GST and TEV cleavage site

    Expression

    Insect

    Mutation

    Note that the first AA in TEV site is R, not E

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFBD-Akt1(WT)

    Plasmid

    #86561

    Purpose

    Expresses human full-length Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

    Plasmid

    #192406

    Purpose

    To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Turbo GFP
    

    Use

    Synthetic Biology

    Tags

    N7

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. IMPT-11371

    Plasmid

    #236191

    Purpose

    Express GPR119 in insect cells

    Depositor

    Vadim Cherezov

    Article

    Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

    Insert

    GPR119 (GPR119 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xMS-TR-100-PURO HRD

    Plasmid

    #207582

    Purpose

    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    

    Tags

    3xMS2

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. RcSxph E540A

    Plasmid

    #194019

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540A

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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