We narrowed to 1,598 results for: Tef

1. CL20_mEGFP_GFM1_MLF1

   Plasmid

   #205825

   Purpose

   Express mEGFP-tagged fusion protein, GFM1_MLF1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   GFM1_MLF1 (GFM1, MLF1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-N-Myc_DW164AA 1-205 USP7

   Plasmid

   #131254

   Purpose

   Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

   Plasmid

   #131255

   Purpose

   Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

   Plasmid

   #131256

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmTHC_PGKneoLox2DTA.2

   Plasmid

   #112624

   Purpose

   H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Human, Anthozoa)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   mTeal (mTFP1)

   Expression

   Mutation

   Promoter

   No Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEHC_PGKneoLox2DTA.2

   Plasmid

   #112623

   Purpose

   H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Emerald (H2BC3 Human, Aequorea victoria)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Emerald

   Expression

   Mutation

   Promoter

   No Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3_N-DYK_MTHFD1-NLS

   Plasmid

   #133023

   Purpose

   NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

   Depositor

   Stefan Kubicek

   Article

   Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3_N-DYK_MTHFD1-NES

   Plasmid

   #133022

   Purpose

   NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

   Depositor

   Stefan Kubicek

   Article

   Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_ZKSCAN1_Tau10-12K317M

   Plasmid

   #194164

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT (MAPT Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6-ISG-IFN

    Plasmid

    #231488

    Purpose

    Standard for type I interferon signature (6 genes)

    Depositor

    Stefano Volpi

    Article

    Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d

    Inserts

    TBP (TBP Human)
    IFI27 (IFI27 Human)
    IFI44L (IFI44L Human)
    IFIT1 (IFIT1 Human)
    ISG15 (ISG15 Human)
    RSAD2 (RSAD2 Human)
    SIGLEC1 (SIGLEC1 Human)
    HPRT1 (HPRT1 Human)
    18s (RPS18 Human)
    G6PD (G6PD Human)
    

    Use

    Qpcr plasmid standard

    Tags

    Expression

    Mutation

    partial sequence

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

    Plasmid Kit

    #1000000210

    Purpose

    Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

    Depositor

    Vincent Martin

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

12. EasyClone-MarkerFree Vector Set

    Plasmid Kit

    #1000000098

    Purpose

    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

    Depositor

    Irina Borodina

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit

    #1000000236

    Purpose

    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

    Depositor

    Daniel Voytas

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

14. Cultivarium POSSUM Toolkit

    Plasmid Kit

    #1000000234

    Purpose

    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

    Depositor

    Nili Ostrov

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

15. CRISPi Kit

    Plasmid Kit

    #1000000136

    Purpose

    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

    Depositor

    Brigitte Gasser

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

16. ScEnSor Kit

    Plasmid Kit

    #1000000215

    Purpose

    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.

    Depositor

    Lisbeth Olsson

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

17. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit

    #1000000138

    Purpose

    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression

    Depositor

    Elena Caro Bernat

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

18. GoldenPiCS Kit

    Plasmid Kit

    #1000000133

    Purpose

    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites

    Depositor

    Brigitte Gasser

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only