We narrowed to 5,078 results for: mcs

1. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

   Plasmid

   #182353

   Purpose

   GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXJ-His-PDCD1LG2

   Plasmid

   #232660

   Purpose

   transient expresses PD-L2 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   PDCD1LG2 (PDCD1LG2 Human)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lyso-RA

   Plasmid

   #209875

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA6A-EGFR ICD (645-1186)

   Plasmid

   #42667

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR ICD (645-1186) (EGFR Human)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pMXs-NKX2.2

   Plasmid

   #134670

   Purpose

   Retroviral expression of mouse Nkx2.2

   Depositor

   Marius Wernig

   Article

   Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

   Insert

   Nkx2.2 (Nkx2-2 Mouse)
   

   Use

   Retroviral

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. human MTAP

   Plasmid

   #64077

   Purpose

   Expression of human 5'-methylthioadenosine phosphorylase

   Depositor

   Vern Schramm

   Article

   Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

   Insert

   5'-methylthioadenosine phosphorylase (MTAP Human, Synthetic)
   

   Tags

   Histidine tag and TEV

   Expression

   Bacterial

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1 Aquamarine

   Plasmid

   #106285

   Purpose

   Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   Aquamarine
   

   Tags

   6xHis and TEV cleavage (N terminal on insert)

   Expression

   Bacterial

   Mutation

   T65S and H148G

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOLA banana sensor sfGFP

   Plasmid

   #107367

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   banana-detecting toehold + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Promoter

   Ubc

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 MDM2 S166D

    Plasmid

    #16234

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Mutation

    Ser166 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHLP710

    Plasmid

    #203740

    Purpose

    Integration of OsTIR1-3xMyc at NEUT5L locus

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    OsTIR1
    

    Use

    Candida albicans integrating vector

    Tags

    3xMyc

    Promoter

    ACT1 (C. albicans)

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 MDM2 S186D

    Plasmid

    #16235

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Mutation

    Ser186 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pMM2-hPMCA2w/b

    Plasmid

    #47583

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM2-hPMCA2z/b

    Plasmid

    #47581

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2z/b (ATP2B2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS425ADS

    Plasmid

    #20119

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene synthase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194385

    Purpose

    Gateway Destination vector for split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-NTEV-TCS-GV-2xHA
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-TRIM28deltaCC2

    Plasmid

    #124957

    Purpose

    Mammalian expression of Human TRIM28 with deleted CC domain

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of CC domains

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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