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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_BFP_2A-mIL10

Plasmid

#247507

Purpose

Expresses mouse IL-10 and BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

mouse IL-10 and BFP constitutive (Il10 Synthetic, Mouse)

Use

Lentiviral

Tags

bfp

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-mEGFP-Actin

Plasmid

#119870

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

beta Actin (Actb Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TO-NGN2-EMX1 BSD-mApple

Plasmid

#182311

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
bsd-T2A-mycNLS-mApple

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EBFP2-nuc

Plasmid

#36085

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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LifeAct-mNeonGreen

Plasmid

#98877

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LifeAct

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VPS4-E228Q (VPS4A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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