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We narrowed to 6,511 results for: GCA;

Showing: 1601 - 1620 of 6511 results

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pYJ34-PB-CRISPR-BANCR-E1-gRNA

Plasmid

#131077

Purpose

long non-coding RNA BANCR knock out

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Insert

BANCR Exon1 gRNA (BANCR Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-mU6-GFPGO2-IRES-mScarletI

Plasmid

#136896

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2-IRES-mScarletI

Use

Lentiviral

Tags

2xNLS on GFP, 3xNLS on mScarlet

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(2)

Plasmid

#73555

Purpose

vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pKL001

Plasmid

#98917

Purpose

Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

CaPR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23118 - Strong Constitutive

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJ23119-sgRNA

Plasmid

#113654

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter J23119 and sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe neo TGFBR3-HA

Plasmid

#83095

Purpose

Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

TGFBR3 (TGFBR3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
LEP-shREN

Plasmid

#105580

Purpose

retrovirally express control shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUBA5

Plasmid

#86132

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UBA5 (UBA5 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern/EGFP

Plasmid

#179913

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PLKO.1-CAPRIN1-CDS

Plasmid

#136054

Purpose

CAPRIN1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTCAGCAGAACACTGGATTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shTGFBR3 puro

Plasmid

#58696

Purpose

Lentiviral shRNA vector for knockdown of human TGFBR3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shTGFBR3 (TGFBR3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Use

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'UAG)

Plasmid

#170126

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'UAG)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-Pdha1

Plasmid

#175936

Purpose

knockout mouse Pdha1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Pdha1 gRNA (Pdha1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-OSBP

Plasmid

#32495

Depositor

Alex Toker

Article

Nhek et al Mol Biol Cell. 2010 Jul 1;21(13):2327-37

Insert

OSBP (OSBP Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR sgSHMT1_1

Plasmid

#106311

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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