We narrowed to 2,386 results for: PUC19

1. pILGFP10CF71

   Plasmid

   #185860

   Purpose

   Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT15484_HF-ABE8e

   Plasmid

   #174122

   Purpose

   Expresses ABE8e fused with SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   HF-ABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT9992-HF-ABE

   Plasmid

   #163000

   Purpose

   plasmid expressing HF-ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   HF-ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9994-HypaR661A-ABE

   Plasmid

   #163002

   Purpose

   plasmid expressing HypaR661A-ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   Hypa-ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   R661A

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT9996-HeF-ABE

   Plasmid

   #163004

   Purpose

   plasmid expressing HeF-ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   HeF-ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL0-NbPT5bPro

   Plasmid

   #159416

   Purpose

   Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   NbPT5b Promoter
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL0-NbBCP1bPro

   Plasmid

   #159417

   Purpose

   Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   NbBCP1b Pro
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL0-MjcDOPA5GT

   Plasmid

   #162530

   Purpose

   Mirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   cDOPA5GT
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTX1-SMN2-exon7-ESE1

   Plasmid

   #126042

   Purpose

   In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   SMN2 exon7 ESE1
   

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PHY55-mouse U6-sgLMNA

    Plasmid

    #164045

    Purpose

    U6-driven sgRNA targeting LMNA

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    LMNA targeting sgRNA
    

    Use

    CRISPR

    Promoter

    mouse U6

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbc

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

    Plasmid

    #149347

    Purpose

    Mammalian expression vector for SpyCas9 and puromycin resistance

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOXA3-donor

    Plasmid

    #112321

    Purpose

    CRISPR donor plasmid to tag human transcription factor FOXA3 with GFP

    Depositor

    Kevin White

    Insert

    FOXA3 homology arms flanking EGFP-IRES-Neo cassette (FOXA3 Human)
    

    Use

    CRISPR

    Mutation

    hg38:19:45872401:G>T, hg38:19:45872421:C>T,…

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN220

    Plasmid

    #91681

    Purpose

    Express sgRNA targeting human VRK2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    VRK2 (VRK2 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN221

    Plasmid

    #91682

    Purpose

    Express sgRNA targeting human VRK2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    VRK2 (VRK2 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN154

    Plasmid

    #91683

    Purpose

    Express sgRNA targeting human ZNF536

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    ZNF536 (ZNF536 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPN155

    Plasmid

    #91684

    Purpose

    Express sgRNA targeting human ZNF536

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    ZNF536 (ZNF536 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP1298‐1

    Plasmid

    #87248

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFPJ1

    Plasmid

    #83561

    Purpose

    Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER signal peptide
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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