We narrowed to 4,299 results for: pme

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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYS16

Plasmid

#202082

Purpose

Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

CT mNG-T2A-PuroR Minicircle Parent

Use

CRISPR

Tags

mNeonGreen

Promoter

None

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp1b-mCherry-stop; cmlc2:GFP

Plasmid

#213739

Purpose

Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp1b-mCherry

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-dTomato-GCaMP6s

Plasmid

#99864

Purpose

expresses dTomato and GCaMP6s under control of the Tet-off system

Depositor

Venkatesh Murthy

Article

Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

Insert

dTomato, GCaMP6s

Promoter

Tet-responsive promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Promoter

CMV

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PKNOX1_pCSdest

Plasmid

#53899

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PKNOX1 (PKNOX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45a IRES GFP

Plasmid

#17880

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45a (Phf10 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pNeuroD1-ERT2CreERT2

Plasmid

#237402

Purpose

Tamoxifen-dependent expression of ERT2CreERT2 in NeuroD1-positive cells

Depositor

Yusuke Kishi

Article

Sakai et al Development. 2025 Jul 1;152(13):dev20456

Insert

ERT2CreERT2

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Expression

Mammalian

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-THT

Plasmid

#55790

Purpose

GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Type

Empty backbone

Use

RNAi; Gateway

Expression

Mammalian

Promoter

modified H1 RNA gene

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTN14

Plasmid

#178945

Purpose

Expresses AtTIR1(F79G) in C elegans

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

AtTIR1(F74G)

Expression

Worm

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Scrambled control shRNA

Plasmid

#40625

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Scrambled control shRNA

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Promoter

Beta Actin

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TMPRSS15_pCSdest

Plasmid

#53806

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TMPRSS15 (TMPRSS15 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
cfSGFP2-N

Plasmid

#37533

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

cysteine-free SGFP2-N

Expression

Mammalian

Mutation

C48S and C70M mutations relative to SGFP2

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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