We narrowed to 5,010 results for: puc

1. pMIR218-2_nt5G>U

   Plasmid

   #174009

   Purpose

   miR218 nt5 point mutation in SLIT3

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   SLIT3 (SLIT3, MIR218-2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NT5 G>U point mutation in SLIT3

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITGA5_Q853L

   Plasmid

   #174008

   Purpose

   Fibronectin receptor binding site point mutation

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   FibronR ITGA5 point mutation (ITGA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   AmpR

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LMPd Amt OXCT1

   Plasmid

   #209407

   Purpose

   Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

   Depositor

   Russell Jones

   Article

   Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

   Insert

   Oxct1 shRNA (Oxct1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rab11 single guide RNA

   Plasmid

   #229683

   Purpose

   guide RNA for Rab11 tagging at the N-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   Rab11 guide (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

   Plasmid

   #229678

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold, HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP1muA-mStayGold-polyA-Hygro HR

   Plasmid

   #229679

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AP1muA-Halo-ALFA-polyA-G418 HR

   Plasmid

   #229681

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Halo, ALFA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

   Plasmid

   #229676

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Halo, 3xALFA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. scQers promoter control #1 (low: minimal promoter, p027)

    Pooled Library

    #217420

    Purpose

    scQers promoter control #1 (low: minimal promoter, p027)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

12. scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Pooled Library

    #217424

    Purpose

    scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

13. scQers promoter control #2 (low: no promoter, p029)

    Pooled Library

    #217421

    Purpose

    scQers promoter control #2 (low: no promoter, p029)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

14. scQers promoter control #3 (intermediate: UBC promoter, p041)

    Pooled Library

    #217422

    Purpose

    scQers promoter control #3 (intermediate: UBC promoter, p041)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

15. scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

    Pooled Library

    #217423

    Purpose

    scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

16. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid

    #197511

    Purpose

    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and BPNLS-NpuC(intein)

    Expression

    Mutation

    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

    Promoter

    Cbh

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Mutation

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. DinoIII-neo

    Plasmid

    #155026

    Purpose

    Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    amino 3'-glycosyl phosphotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. DinoIII-pat

    Plasmid

    #155028

    Purpose

    Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Phosphinothricin N-acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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