Addgene

We narrowed to 6,948 results for: 11

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Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Article

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NFIA1.1

Plasmid

#112698

Purpose

Expression of NFIA1.1 protein for transient transfection assays

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

Insert

Nuclear Factor I A1.1 (Nfia Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mUbp43

Plasmid

#12454

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (Usp18 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3R1

Plasmid

#174191

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-ANXA7-mRuby3

Plasmid

#184893

Purpose

Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA7 (ANXA7 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-C1(2)delta

Plasmid

#21216

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-N2-PKCdelta-C1 (Prkcd Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

C1(2) domains of PKC delta (rat) (Lab plasmid WC…

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMZ3012

Plasmid

#204400

Purpose

A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator rhlR (rhlR )
GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PftsH and Synthetic promoter PRhl0L0L12

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pC0090

Plasmid

#181954

Purpose

Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xTOPFlash

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pIntegrase_9

Plasmid

#60580

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_9

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-GFP-NMHC II-A

Plasmid

#10844

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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UnReg-H2B-YFP-DD

Plasmid

#96892

Purpose

Reporter to monitor translation of unstructured mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

UnReg-H2B-Venus-DD

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLoxP-DHFR-mCherry

Plasmid

#70147

Purpose

Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

Depositor

David Sibley

Article

Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

Insert

a selectable dihydrofolate reductase-thymidylate synthase marker

Use

Expression in toxoplasma gondii

Tags

mCherry

Expression

Mutation

Promoter

DHFR 5' UTR

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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