We narrowed to 2,891 results for: 974

1. pDONR223 GABARAP no-stop

   Plasmid

   #123209

   Purpose

   Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Stop codon removed

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGB2alpha2 35s:hCas9:tNos (GB0639)

   Plasmid

   #68222

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV 3xFLAG-GABARAP G116A

   Plasmid

   #123098

   Purpose

   Expresses 3xFLAG-GABARAP G116A in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98976

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(N863A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FusionRed-Sit-N-15

   Plasmid

   #56133

   Purpose

   Localization: Golgi TGN, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   Sit (ST6GAL1 Human)
   

   Use

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. TAL2238

   Plasmid

   #36689

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD7-TALEN_Left (Chd7 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. TAL2239

   Plasmid

   #36690

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD7-TALEN_Right (Chd7 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-Flag-YAP-S61A

   Plasmid

   #33087

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S61A

   Promoter

   CMV

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223 GABARAP G116

   Plasmid

   #123082

   Purpose

   Gateway entry clone encoding human GABARAP G116

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJC6-Flag CASTOR1 I280A

    Plasmid

    #84497

    Purpose

    Lentiviral expression of CASTOR2 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Flag-YAP-S381A

    Plasmid

    #33090

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S381A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pLacIBD::SDRX (GB0673)

    Plasmid

    #68183

    Purpose

    Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    LacI Binding Domain fused to SRDX Transcriptional Repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRK5-HA CASTOR1 ACT2

    Plasmid

    #84492

    Purpose

    overexpression of CASTOR1 ACT2 domain (aa 169-end)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 ACT2 (GATSL3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    169-last amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-Flag CASTOR1 ACT1

    Plasmid

    #84491

    Purpose

    overexpression of CASTOR1 ACT1 domain (aa 1-169)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 ACT1 (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-169 amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CS2-Myc-mASUN

    Plasmid

    #47044

    Purpose

    Expression of tagged-mouse ASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

    Insert

    Asunder (Asun Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-Flag-YAP-H122L

    Plasmid

    #33086

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H122L

    Promoter

    CMV

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

20. psiCHECK RBJ 3'UTR

    Plasmid

    #19866

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    RBJ 3'UTR (DNAJC27 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge