We narrowed to 32,487 results for: Eng;

1. pX330a dCas9-VP64

   Plasmid

   #92363

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcU6_1 sgRNA

   Plasmid

   #92395

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.1 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.1

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI Cas9 H2B-RFP

   Plasmid

   #92397

   Purpose

   CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcg 2.0-BFP-circ-arRNA backbone

   Plasmid

   #213965

   Purpose

   pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pminiTol2-HuC-NE2h

   Plasmid

   #208689

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   Cloning vector

   Promoter

   Huc

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1352wUV2 F2bbs kan GCANNNAGGk

   Plasmid

   #18752

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1352wUV2 F3bbs kan NNNTGGGCGk

   Plasmid

   #18756

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1352wUV2 F1bbs kan GCGTGGNNN

    Plasmid

    #18753

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128342

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna7 (Chrna7 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TDK promoter dCas9

    Plasmid

    #113657

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    TDK gene promoter and dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TDK gene promoter

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-EGFP-APOBEC1-YTHmut

    Plasmid

    #209320

    Purpose

    AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

    Depositor

    Magdalena Koziol

    Article

    Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

    Insert

    APOBEC1-YTHmut-HA
    

    Use

    AAV

    Tags

    HA

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pminiTol2-HuC-NE2m

    Plasmid

    #208688

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-dt/deltaEGFP

    Plasmid

    #163919

    Purpose

    PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    dtomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CBX1mut-Dronpa

    Plasmid

    #138250

    Purpose

    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

    Depositor

    Jin Zhang

    Article

    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

    Insert

    CBX1mut-Dronpa
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Native promoter dCas9

    Plasmid

    #113656

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    Native promoter and dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    S. pyogenes Cas9 native promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SuExp-hPLD3

    Plasmid

    #201243

    Purpose

    express full length human PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (PLD3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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