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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Luc-T20

Plasmid

#177941

Purpose

Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

Luc2 (ORF1ab )

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Luc-T20

Plasmid

#177941

Purpose

Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

Luc2 (ORF1ab )

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-EF1a-D614G-N501Y

Plasmid

#177939

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-EF1a-D614G-N501Y

Plasmid

#177939

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3h

Plasmid

#208699

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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