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pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Publication

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1_CD

Plasmid

#83570

Purpose

Expresses the catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Publication

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mEmerald-Caveolin-N-10

Plasmid

#54026

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles
pENTR-MECP2

Plasmid

#47806

Purpose

Expresses methyl-CpG binding protein 2 (Rett Syndrome)

Depositor

Huda Zoghbi

Publication

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Gateway Cloning

Mutation

wild type

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Stat3-SH2

Plasmid

#46513

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat3 (STAT3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 584-700

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKO (Kan)

Plasmid

#110086

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Publication

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Publication

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Expression

Mammalian

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSIM24-GUS

Plasmid

#67882

Purpose

Plant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

Depositor

Indu Maiti

Publication

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

uidA

Expression

Plant

Mutation

T120A

Promoter

M24

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTet-O-Nd1-puro

Plasmid

#52052

Purpose

Expresses Nd1 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Publication

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabe-Puro-Flag-IKBKE

Plasmid

#15292

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pLVX-opto-hCaspase-9

Plasmid

#208787

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 9 (CASP9 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-1304)

Plasmid

#74244

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Publication

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-1304

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCXN2-mVIN

Plasmid

#11776

Depositor

Eileen Adamson

Publication

Xu et al J Cell Sci. 1998 Jun;111 ( Pt 11):1535-4

Insert

vinculin (Vcl Mouse)

Expression

Mammalian

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
pMPRAv3:minP-GFP

Plasmid

#109036

Purpose

MPRA v3 minimal promoter with GFP donor

Depositor

Pardis Sabeti, Ryan Tewhey

Publication

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Insert

emGFP

Expression

Mammalian

Promoter

minP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA_B2AR-s-pep

Plasmid

#47438

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pC3.1-caggs-Crimson-CAAX

Plasmid

#190041

Purpose

Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Depositor

Michael Lin

Publication

Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

Insert

Crimson

Tags

CAAX

Expression

Mammalian

Promoter

caggs

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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