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Showing: 1621 - 1640 of 16067 results

  7. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65769-plasmid-map-sequence-id-112659

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  8. p2R3z-BASI-ccdB-BSAI

    Plasmid
    #118389
    Purpose
    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Publication
    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4
    Insert
    ccdB
    Use
    Unspecified
    Mutation
    See Depositor Comments Section
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118389-plasmid-map-sequence-id-470418

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  9. pGR

    Plasmid
    #46002
    Purpose
    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose
    Depositor
    Christopher Voigt
    Publication
    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available since
    June 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46002-plasmid-map-sequence-id-64680

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  11. pTriEx-mCherry::LANS4

    Plasmid
    #60785
    Purpose
    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation
    Depositor
    Brian Kuhlman
    Publication
    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LANS4
    Tags
    mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60785-plasmid-map-sequence-id-118599

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  18. pSBbi_RP_lbMicroID2-HA

    Plasmid
    #188522
    Purpose
    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lbMicroID2
    Use
    Transposon mediated gene integration
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188522-plasmid-map-sequence-id-372813

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  19. pIRES-hrGFP II-TET1_CD

    Plasmid
    #83570
    Purpose
    Expresses the catalytic domain of TET1 in mammalian cells
    Depositor
    Jean-Pierre Issa
    Publication
    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Tags
    3X FLAG tag and hr GFP II
    Expression
    Mammalian
    Mutation
    TET1 amino acids 1-1417 deleted
    Promoter
    CMV
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83570-plasmid-map-sequence-id-191046

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Showing: 1621 - 1640 of 16067 results