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Showing: 1621 - 1640 of 8465 results

  2. PX458 eSp(1.1) V3

    Plasmid
    #226963
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226963-plasmid-map-sequence-id-450822

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  3. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  4. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  5. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

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  6. Pdg459-eSp(1.1) V3

    Plasmid
    #226965
    Purpose
    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226965-plasmid-map-sequence-id-450820

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  7. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  8. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  9. pBHM2644

    Plasmid
    #229528
    Purpose
    Carries AID* tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229528-plasmid-map-sequence-id-459927

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  10. pBHM2645

    Plasmid
    #229529
    Purpose
    Carries mIAA7_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mIAA7_opt - empty sgRNA - NATR
    Use
    CRISPR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229529-plasmid-map-sequence-id-459936

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  11. pBHM2650

    Plasmid
    #229992
    Purpose
    Carries AID* tag, empty sgRNA, and HYGR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - HYGR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229992-plasmid-map-sequence-id-461779

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  12. pBHM2652

    Plasmid
    #229994
    Purpose
    Carries AID* tag, empty sgRNA, and amdS marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - amdS
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229994-plasmid-map-sequence-id-461120

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  13. pDECKO_GFP_AAVS1_186

    Plasmid
    #159091
    Purpose
    targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    159091-plasmid-map-sequence-id-308784

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  14. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  15. gRNA telo/GFP

    Plasmid
    #137713
    Purpose
    Vector co-expressing sgRNA telo and GFP
    Depositor
    Dominik Niopek
    Insert
    sgRNA telo + GFP
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6/RSV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    137713-plasmid-map-sequence-id-272438

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  16. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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Showing: 1621 - 1640 of 8465 results