We narrowed to 9,373 results for: Pol;

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pPK1E-1

Plasmid

#48145

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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D-raf G621S

Plasmid

#39769

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

G621S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf C295S

Plasmid

#39768

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf F290I

Plasmid

#39766

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

F290I

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+S620A

Plasmid

#39765

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and S620A

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+C295S

Plasmid

#39764

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pET-28a_6H-TAQ_E602D

Plasmid

#166944

Purpose

Bacterial expression of Taq DNA polymerase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

Thermus aquaticus DNA polymerase

Tags

6X His

Expression

Bacterial

Mutation

E602D

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD

Plasmid

#235577

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFEndoNA2

Plasmid

#174667

Purpose

Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA2 fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRi_Mxi1_yl_NHEJ

Plasmid

#91249

Purpose

CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

Inserts

Codon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Kmt5cFL

Plasmid

#235581

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

Plasmid

#235586

Purpose

Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Mutation

G282A

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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eDrIR1

Plasmid

#183998

Purpose

Optically controlled eDrIR1 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrIR1 (INSR Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCCM’

Plasmid

#162709

Purpose

pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Mutation

p15A origin replaced with high-copy colE1 origin

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

GPDp

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEAK-Flag/INPP4B

Plasmid

#24324

Depositor

Lewis Cantley

Article

Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

Insert

Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

none

Available Since

May 11, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 wt (Nigg RG6)

Plasmid

#41160

Depositor

Erich Nigg

Article

Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-LAMP1

Plasmid

#168513

Purpose

Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

LAMP1 (Lamp1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Tlr4

Plasmid

#27148

Depositor

Bruce Beutler

Article

Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

Insert

toll-like receptor 4 (Tlr4 Mouse)

Tags

3x FLAG

Expression

Mammalian

Mutation

Native signal peptide sequence (original amino ac…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

Plasmid

#235590

Purpose

Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Mutation

NHDC182-185AAAG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

Plasmid

#195536

Purpose

Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR-Kv2.1PR

Plasmid

#195525

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1 M161A/D165K

Plasmid

#65809

Purpose

expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC3 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

M161A/D165K

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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